Publications

2026 Programming T cells for intercellular genome editing Kai Chen · bioRxiv, 2026.06 · Scholar only — 2025 Directed evolution expands CRISPR-Cas12a genome editing capacity Kai Chen · Nucleic Acids Research 53 (13), gkaf649 · Scholar only 23 2025 Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR–Cas9 ribonucleoprotein Kai Chen · Nature Biotechnology · Scholar only 217 2024 Engineering self-deliverable ribonucleoproteins for genome editing in the brain Kai Chen · Nature Communications · Scholar only 61 2024 Enzymatic assembly of diverse lactone structures: An intramolecular C–H functionalization strategy Kai Chen · Journal of the American Chemical Society · Scholar only 30 2024 Rapid DNA unwinding accelerates genome editing by engineered CRISPR–Cas9 Kai Chen · Cell 187 (13), 3249-3261. e14 · Scholar only 84 2023 Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes Kai Chen · Nucleic acids research · Scholar only 43 2023 Reversing the enantioselectivity of enzymatic carbene N–H insertion through mechanism‐guided protein engineering Kai Chen · Angewandte Chemie International Edition · Scholar only 34 2022 Improved genome editing by an engineered CRISPR-Cas12a Kai Chen · Nucleic acids research · Scholar only 115 2021 A method for enantioselective carbenoid C–H insertion using an iron-containing protein catalyst Kai Chen · US Patent 10,934 · Scholar only 1 2021 Diverse carbene transferase enzyme catalysts derived from a P450 enzyme Kai Chen · US Patent 20,210,292 · Scholar only — 2021 Dual-function enzyme catalysis for enantioselective carbon–nitrogen bond formation Kai Chen · Nature Chemistry · Scholar only 101 2021 Engineering enzymes for new-to-nature carbene chemistry Kai Chen · Transition Metal-Catalyzed Carbene Transformations · Scholar only 6 2021 Method for producing an organosilicon product Kai Chen · US Patent 10,927 · Scholar only — 2020 Biocatalytic synthesis of strained carbocycles Kai Chen · US Patent 10,829 · Scholar only 2 2020 Engineering cytochrome P450s for enantioselective cyclopropenation of internal alkynes Kai Chen · Journal of the American Chemical Society · Scholar only 104 2020 Engineering new catalytic activities in enzymes Kai Chen · Nature Catalysis · Scholar only 974 2020 Enzymatic lactone-carbene C‒H insertion to build contiguous chiral centers Kai Chen · ACS Catalysis · Scholar only 61 2020 Heme protein catalysts for carbon–boron bond formation in vitro and in vivo Kai Chen · US Patent 10,745 · Scholar only — 2019 Directed evolution of a cytochrome P450 carbene transferase for selective functionalization of cyclic compounds Kai Chen · Journal of the American Chemical Society · Scholar only 171 2019 Engineered cytochrome c-catalyzed lactone-carbene B–H insertion Kai Chen · Synlett · Scholar only 45 2019 Enzymatic assembly of carbon–carbon bonds via iron-catalysed sp³ C–H functionalization Kai Chen · Nature · Scholar only 382 2018 Alternate heme ligation steers activity and selectivity in engineered cytochrome P450-catalyzed carbene transfer reactions Kai Chen · Journal of the American Chemical Society · Scholar only 133 2018 Cytochrome c protein variants for catalyzing carbon-silicon bond formation Kai Chen · US Patent 10,093 · Scholar only 2 2018 Diverse engineered heme proteins enable stereodivergent cyclopropanation of unactivated alkenes Kai Chen · ACS Central Science · Scholar only 162 2018 Enzymatic construction of highly strained carbocycles Kai Chen · Science · Scholar only 270 2018 Macrocyclic peptide construction through C–H activation strategy Kai Chen · Science Bulletin · Scholar only 11 2018 Stereoselective enzymatic synthesis of heteroatom-substituted cyclopropanes Kai Chen · ACS Catalysis · Scholar only 115 2017 Genetically programmed chiral organoborane synthesis Kai Chen · Nature · Scholar only 348 2017 Heme protein catalysts for carbon-silicon bond formation in vitro and in vivo Kai Chen · US Patent App · Scholar only 2 2017 Palladium-catalyzed interannular meta-C–H arylation Kai Chen · Chemical Communications · Scholar only 43 2017 Palladium-catalyzed sequential monoarylation/amidation of C(sp³)–H bonds: stereoselective synthesis of α-amino-β-lactams and anti-α,β-diamino acid Kai Chen · Chemical Communications · Scholar only 47 2016 Directed evolution of cytochrome c for carbon–silicon bond formation: Bringing silicon to life Kai Chen · Science · Scholar only 729 2016 Divergent and stereoselective synthesis of β-silyl-α-amino acids through palladium-catalyzed intermolecular silylation of unactivated primary and secondary C‒H bonds Kai Chen · Angewandte Chemie International Edition · Scholar only 149 2016 Palladium-catalyzed C(sp³)–H arylation of lactic acid: Efficient synthesis of chiral β-aryl-α-hydroxy acids Kai Chen · Organic Chemistry Frontiers · Scholar only 15 2016 Stereoselective alkoxycarbonylation of unactivated C(sp³)–H bonds with alkyl chloroformates via Pd(II)/Pd(IV) catalysis Kai Chen · Nature communications · Scholar only 74 2016 Synthesis of chiral α-hydroxy acids via palladium-catalyzed C(sp³)–H alkylation of lactic acid Kai Chen · Chemical Communications · Scholar only 29 2015 Palladium-catalyzed arylation of unactivated γ-methylene C(sp³)‒H and δ-C‒H bonds with an oxazoline-carboxylate auxiliary Kai Chen · Chemistry-A European Journal · Scholar only 66 2015 Pd(II)-catalyzed direct sulfonylation of unactivated C(sp³)–H bonds with sodium sulfinates Kai Chen · Organic Letters · Scholar only 123 2015 Practical synthesis of anti-β-hydroxy-α-amino acids by Pd(II)-catalyzed sequential C(sp³)‒H functionalization Kai Chen · Chemistry-A European Journal · Scholar only 67 2015 Stereoselective synthesis of chiral β-fluoro-α-amino acids via Pd(II)-catalyzed fluorination of unactivated methylene C(sp³)–H bonds: Scope and mechanistic studies Kai Chen · Journal of the American Chemical Society · Scholar only 209 2014 A general and practical palladium-catalyzed monoarylation of β-methyl C(sp³)–H of alanine Kai Chen · Chemical Communications · Scholar only 89 2014 Recent progress in the synthesis of functionalized β-lactams through transition-metal-catalyzed C(sp³)–H amidation Kai Chen · Synlett · Scholar only 50 2014 Sulfonamide-promoted palladium(II)-catalyzed alkylation of unactivated methylene C(sp³)‒H bonds with alkyl iodides Kai Chen · Angewandte Chemie International Edition · Scholar only 156 2013 Pd(II)-catalyzed alkoxylation of unactivated C(sp³)–H and C(sp²)–H bonds using a removable directing group: Efficient synthesis of alkyl ethers Kai Chen · Chemical Science · Scholar only 331 2013 Pd(II)-catalyzed alkylation of unactivated C(sp³)–H bonds: Efficient synthesis of optically active unnatural α-amino acids Kai Chen · Chemical Science · Scholar only 230 2013 Stereoselective synthesis of chiral α-amino-β-lactams through palladium(II)-catalyzed sequential monoarylation/amidation of C(sp³)‒H bonds Kai Chen · Angewandte Chemie International Edition · Scholar only 343 2012 Synthesis of benzoxazine and 1,3-oxazine derivatives via ligand-free copper(I)-catalyzed one-pot cascade addition/cyclization reaction Kai Chen · Tetrahedron · Scholar only 27