Publications

2026 A GUV-based assay to reconstitute membrane tethering in vitro Michael J. Ragusa · Molecular biology of the cell · Scholar only — 2026 Atg23 interacts with both the N-and C-termini of Atg9 via a hydrophobic binding pocket Michael J. Ragusa · Journal of Biological Chemistry · Scholar only 1 2026 Determinants of protein phosphatase 1β substrate specificity for MyPhoNE motif-containing proteins Michael J. Ragusa · Journal of Biological Chemistry · Scholar only — 2026 Membrane composition-dependent patterning of Rho and F-actin in an artificial cell cortex Michael J. Ragusa · Molecular Biology of the Cell · Scholar only — 2026 Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics Michael J. Ragusa · Autophagy · Scholar only 5 2026 The Z-shaped N-terminal Domain of Atg11 Coordinates Atg9 Recruitment in Selective Autophagy Michael J. Ragusa · bioRxiv, 2026.08 · Scholar only — 2025 The LC3-interacting region of NBR1 is a protein interaction hub enabling optimal flux Michael J. Ragusa · Journal of Cell Biology · Scholar only 11 2025 The rapidly expanding role of LC3-interacting regions in autophagy Michael J. Ragusa · Journal of Cell Biology · Scholar only 16 2025 The structure of a NEMO construct engineered for screening reveals novel determinants of inhibition Michael J. Ragusa · Structure 33 (4), 691-704. e6 · Scholar only 2 2024 Conserved C-Terminal Tail Is Responsible for Membrane Localization and Function of Pseudomonas aeruginosa Hemerythrin Michael J. Ragusa · Biochemistry · Scholar only 6 2024 Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa Michael J. Ragusa · Nucleic Acids Research · Scholar only 1 2024 The structure of the diheme cytochrome c4 from Neisseria gonorrhoeae reveals multiple contributors to tuning reduction potentials Michael J. Ragusa · Journal of inorganic biochemistry · Scholar only 2 2023 A comparative analysis of the membrane binding and remodeling properties of two related sorting nexin complexes involved in autophagy Michael J. Ragusa · Biochemistry · Scholar only 10 2022 Atg23 is a vesicle-tethering protein Michael J. Ragusa · Autophagy · Scholar only 1 2022 Characterization of Protein–Membrane Interactions in Yeast Autophagy Michael J. Ragusa · Cells · Scholar only 6 2022 Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy Michael J. Ragusa · Cell reports · Scholar only 22 2021 A highly conserved glutamic acid in ALFY inhibits membrane binding to aid in aggregate clearance Michael J. Ragusa · Traffic · Scholar only 13 2021 Membrane binding and homodimerization of Atg16 via two distinct protein regions is essential for autophagy in yeast Michael J. Ragusa · Journal of molecular biology · Scholar only 33 2020 Structure and redox properties of the diheme electron carrier cytochrome c4 from Pseudomonas aeruginosa Michael J. Ragusa · Journal of inorganic biochemistry · Scholar only 16 2020 The carboxy terminus of yeast Atg13 binds phospholipid membrane via motifs that overlap with the Vac8-interacting domain Michael J. Ragusa · Autophagy · Scholar only 24 2020 The third coiled coil domain of Atg11 is required for shaping mitophagy initiation sites Michael J. Ragusa · Journal of molecular biology · Scholar only 18 2019 Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO Michael J. Ragusa · JoVE (Journal of Visualized Experiments) · Scholar only 3 2019 The IKK-binding domain of NEMO is an irregular coiled coil with a dynamic binding interface Michael J. Ragusa · Scientific Reports · Scholar only 38 2019 The structure of a highly-conserved picocyanobacterial protein reveals a Tudor domain with an RNA-binding function Michael J. Ragusa · Journal of Biological Chemistry · Scholar only 3 2018 An atypical BAR domain protein in autophagy Michael J. Ragusa · Autophagy · Scholar only 7 2018 A pseudo-receiver domain in Atg32 is required for mitophagy Michael J. Ragusa · Autophagy · Scholar only 24 2017 Backbone and side chain resonance assignments for a structured domain within Atg32 Michael J. Ragusa · Biomolecular NMR assignments · Scholar only 4 2017 Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction Michael J. Ragusa · Proceedings of the National Academy of Sciences · Scholar only 55 2015 Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site Michael J. Ragusa · Structure · Scholar only 48 2014 Assembly and dynamics of the autophagy-initiating Atg1 complex Michael J. Ragusa · Proceedings of the National Academy of Sciences · Scholar only 86 2014 The beginning of the end: how scaffolds nucleate autophagosome biogenesis Michael J. Ragusa · Trends in cell biology · Scholar only 101 2013 A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy Michael J. Ragusa · Proceedings of the National Academy of Sciences · Scholar only 165 2013 What the N-terminal domain of Atg13 looks like and what it does: a HORMA fold required for PtdIns 3-kinase recruitment Michael J. Ragusa · Autophagy · Scholar only 11 2012 Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis Michael J. Ragusa · Cell · Scholar only 290 2012 How Atg18 and the WIPIs sense phosphatidylinositol 3-phosphate Michael J. Ragusa · Autophagy · Scholar only 26 2012 Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy Michael J. Ragusa · Molecular cell · Scholar only 249 2011 Flexibility in the PP1: spinophilin holoenzyme Michael J. Ragusa · FEBS letters · Scholar only 26 2011 Molecular Investigations of the Structure and Function of the Protein Phosphatase 1: Spinophilin: Inhibitor-2 Heterotrimeric Complex Michael J. Ragusa · Biochemistry · Scholar only 61 2010 Spinophilin directs protein phosphatase 1 specificity by blocking substrate binding sites Michael J. Ragusa · Nature structural & molecular biology · Scholar only 241 2010 Structural diversity in free and bound states of intrinsically disordered protein phosphatase 1 regulators Michael J. Ragusa · Structure · Scholar only 131 2010 The extended PP1 toolkit: designed to create specificity Michael J. Ragusa · Trends in biochemical sciences · Scholar only 683 2009 GRK2 activation by receptors: role of the kinase large lobe and carboxyl terminal tail Michael J. Ragusa · Biochemistry · Scholar only 41 2008 Transcription factor MEF2C influences neural stem/progenitor cell differentiation and maturation in vivo Michael J. Ragusa · Proceedings of the National Academy of Sciences · Scholar only 342 2007 Structural characterization of the neurabin sterile alpha motif domain Michael J. Ragusa · Proteins: Structure, Function, and Bioinformatics · Scholar only 10 2005 Generation of conditional Mef2cloxP/loxP mice for temporal‐ and tissue‐specific analyses Michael J. Ragusa · genesis · Scholar only 99